GPI anchoring of protein in yeast and mammalian cells or: How we learned to stop worrying and love glycophospholipids

Thematic review series: lipid posttranslational modifications. GPI anchoring of protein in yeast and mammalian cells, or: how we learned to stop worrying and love glycophospholipids.

Glycosylphosphatidylinositol (GPI) anchoring of cell surface proteins is the most complex and metabolically expensive of the lipid posttranslational modifications described to date. The GPI anchor is synthesized via a membrane-bound multistep pathway in the endoplasmic reticulum (ER) requiring >20 gene products. The pathway is initiated on the cytoplasmic side of the ER and completed in the ER ...

GPI-Anchored Fibromodulin as a Novel Target in Chronic Lymphocytic Leukemia: Diagnostic and Therapeutic Implications

Background: We have previously reported the aberrant expression of Fibromodulin (FMOD) in patients with chronic lymphocytic leukemia (CLL). Although FMOD has been considered as a cytoplasmic or secretory protein, we discovered the cell surface expression of FMOD in leukemic B cells via anchoring with glycosylphosphatidylinositol (GPI). Objective: To evaluate FM...

Zornian Functional Analysis or: How I Learned to Stop Worrying and Love the Axiom of Choice

GPI anchoring facilitates propagation and spread of misfolded Sup35 aggregates in mammalian cells.

Prion diseases differ from other amyloid-associated protein misfolding diseases (e.g. Alzheimer's) because they are naturally transmitted between individuals and involve spread of protein aggregation between tissues. Factors underlying these features of prion diseases are poorly understood. Of all protein misfolding disorders, only prion diseases involve the misfolding of a glycosylphosphatidyl...

The future of human - computer interaction , or how I learned to stop worrying and love my intelligent room

interaction, or how I learned to stop worrying and love my intelligent room Michael H. Coen, MIT Artificial Intelligence Lab Predicting the future is notoriously difficult. Suppose 100 years ago someone suggested that every bedroom in the US would soon have a bell that anyone in the world could ring anytime, day or night. Would you have believed it? Nevertheless, the telephone caught on and has...